Introduction
The NanoPro 1000 system uses capillary isoelectric focusing (cIEF) separation to resolve various modification states of signaling proteins, especially phosphorylation states. Samples as few as 400nl is injected, separated, immobilized, detected and quantitated in capillaries automatically. Because of its sensitivity, characterization of signaling proteins may be invetigated directly in scarce samples, such as fine-needle tumor aspirates or primary cells.
For more detail, please visit NanoPro Technology website.
Applications
The capillary-based immunoassay format is extremely sensitive, enabling the accurate detection and measurement of even protein isoforms.
NanoPro 1000 is capable for the following studies:
Oncoprotein Activation Study
Quantification of individual post-translational modifications, including deamidation, C-terminal lysines, glutathionylation, sialylation, oxidation, glycoslyation glycation and any other changes that will result in a pI shift.
Detailed phospho-isoform profiling
Discovery of kinase inhibitor drug effects
Established assays are 4EBP1, 4EBP2, ABCA1, Acetylated Lysine, AKT, AKT(p), AKT1, AKT2, AKT3, ALAS1 (Control), Anti-goat-IgG-HRP, Anti-human-IgG-HRP, ASNS, Beta actin, Beta amyloid, Beta-2-microglobulin (Control), Bim, Caspase 3, cIAP, cIAP1, cMyc, c-Myc epitope tag, COX IV, cRAF(p), CRKL, Crk-L, CRKL(p), eEF2, eEF2(p), EGFR, EGFR(p), ERK 1/2(p), ERK1, ERK1/2, ERK1/2, ERK2, ERp44, FGFR1, FGFR1(p), FLAG, FLAG epitope tag, FOXO3a, FOXO3a(p), GAPDH (Control), GFP, GluR1(p), GRASP55, GSK3-alpha (basic protein), GSK3-alpha (basic protein)(p), GSK3-alpha/beta, GSK3-beta (basic protein), GSK3-beta (basic protein)(p), GSK3£\, GSK3£], HA epitope tag, HA tag, HGF, Histone, Histone H2A, Histone H2A, K5-Acetyl, Histone H2AX, S139-Phospho, Histone H3, HMGB1, Hsc70 (Hsp73), HSP27, HSP70, HSP70 (Control), HSP90, Human Growth Hormone (hGH), IkB, IkB alpha, IkB(p), IKKalpha, IKKalpha/beta(p), IKKbeta, IKKepsilon, IKKepsilon(p), IKKgamma, JNK, JNK(p), Jun(p), LDH, LDHA, LDHB, LMP2, LRP6, Mcl-1, MEK 1/2, MEK1, MEK1(p), MEK1/2(p), MEK2, MEK2(p), MET, MET(p), Methylated Arginine, Mfn 1, Mfn 2, MFN1, MFN2, MK2, MK3, MYC, NDRG1, NDRG1(p), NF-kB, NF-kB(p), p27, p27(p), p38(p), p-4E-BP1, p53, p53(p), p70S6 kinase, PAK1, PAK1(p), PAK1,2,3, PAK1/2, PAK2, PAK3, pEGFR, Phospho-p27/KIP1, Phospho-p38, Phospho-Tyrosine (not specific to EGFR), p-JNK, PKC alpha, PKC delta, PKC delta(p), PLC gamma 1, PLC gamma 1(p), PLC-gamma 1, PRAS40, PSA, p-Stat3, p-Stat5, PTEN, PTEN(p), PTPRC/CD45, RPA2, RPA2(p), S6 Ribosomal Protein, S6 Ribosomal Protein(p), Src, Src(p), STAT1, STAT1(p), STAT3, STAT3(p), STAT5, STAT5(p), Survivin, Thioredoxin 1 (Control), Tubulin alpha, Ubiquitin and VEGF.
Suggested Reading:
NanoPro 1000 System User Guide
NanoPro Assay Development Guide
O'Neill RA, 2006, Isoelectric focusing technology quantifies protein signaling in 25 cells, PNAS, 103, 16153-16158.
Fan AC, 2009, Nanofluidic proteomic assay for serial analysis of
oncoprotein activation in clinical specimens, Nature Medicine, 15, 566-571.
There are more citation may be found in ProteinSimple webpages.
