MicroCal iTC200
iTC 200 is an isothermal titration calorimeter. Isothermal titration Calorimetry is the standard for studying biomolecular interaction, which directly measures the releasing and absorbing of heat during a biomolecular event. The binding affinity (Ka), enthalpy change (ΔH) and binding stoichiometry (n) could be determined in a single experiment.
For more detail, please refer to MicroCal iTC200 brochure.
Important Notification:
*(2024-04-01) iTC200 is out of service beause of slow response of its thermal core which might be caused by contamination from sample precipitates or simply aging of the instrument (16 years old). ITC samples can be submitted to our Automatic PEAQ-ITC. Sorry for the inconvenience.
*(2024-03-11) PEAQ-ITC Automatic system is available for service through BCF reservation system. Please review sample submission guideline before submit your sample.
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(2024-02-29) PEAQ-ITC Automatic system is suffering from loading problem. Service is called. Sorry for the inconvenience.
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(2023-09-25) Please turn on the iTC200 instrument for at least 30 min for stable baseline and consistent injection heat.
*(2023-05-11) PEAQ-ITC Automatic system is available for service through BCF reservation system. Please review sample submission guideline before submit your sample.
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(2022-06-08) PEAQ-ITC Automatic system is down because of an unknow problem. Service is called. Sorry for the inconvenience.
* (2021-07-09) The washing module has been restored from a major demage. It takes two times now to fill the syringe. Please contact BCF staff if you'd like to fill syringe manually.
* (2020-08-10) Due to the increase of the injector syringe cost, we adjust the charge to NT$11,180 for your 1st broken syringe, and NT$44,723 for the 2nd.
* (2019-09-16) iTC200-BCF is down because of PC connection problems. Service is called. Sorry for the inconvenience.
* (2019-03-01) PEAQ-ITC is available for service through BCF reservation system. Please review sample submission guideline before submit your samples.
* (2018-01-01) Free PEAQ-ITC sample tests available for Sinica users only till 2018-02-28.
* Do not shake, bang or knock the bench durining experiments.
* Do not plug or withdraw USB in the middle of a run; this causes system error and the experiment will be aborted by the control program.
* Do not analyze the data file that is not finished. Instead, copy the data file and analyze the copied data.
* The process for cleaning the injector syringe is modified, please ask Dr. Jao for the correct procedure.
* Use only 200uL PCR tubes provided by BCF (Rainin Cat#3-TC02-N). Some PCR tubes (ABgene cat.# AB-0337) do not fit the holder and the injector syringe may hit the bottom of the tubes.
Selected Applications
(Bio)molecular interactions
Dissociations (Dimer)
Interactions with vesicles/micelles
Enzyme kinetics
Related Softwares
Specification
| Cell volume | 200 μl |
| Min. Sample requirement | 5 - 10 μg of protein |
| direct measure range (milli - nano) | 102 to 109 M-1 |
| competetive measure range (nano - pico) | 109 to 1012 M-1 |
Suggested Reading:
iTC200 Microcalorimeter User's Manual.
ITC Data Analysis in Origin Tutorial Guide.
Picchianti, Lorenzo, Arthur Sedivy, and Yasin Dagdas, 2023, Characterization of Atg8-Family Interactors by Isothermal Titration Calorimetry, In Plant Proteostasis: Methods and Protocols, Edited by L. Maria Lois and Marco Trujillo.
Freyer MW and Lewis EA, 2008, Isothermal titration calorimetry: experimental design, data analysis, and probing macromolecule/ligand binding and kinetic interactions, Methods in Cell Biology, 84, 79-113.
Bjelić S and Jelesarov I, 2008, A survey of the year 2007 literature on applications of isothermal titration calorimetry, J. Mol. Recognit., 21, 289-311.
Thomson JA, Ladbury JE, 2004, Isothermal titration calorimetry: A tutorial. In Biocalorimetry 2: Applications of calorimetry in the biological sciences, Ladbury JE, Doyle ML (eds). John Wiley & Sons Ltd.:Chichester, UK; pp.37-58.