MicroCal iTC200
iTC 200 is an isothermal titration calorimeter. Isothermal titration Calorimetry is the standard for studying biomolecular interaction, which directly measures the releasing and absorbing of heat during a biomolecular event. The binding affinity (Ka), enthalpy change (ΔH) and binding stoichiometry (n) could be determined in a single experiment.
For more detail, please refer to MicroCal iTC200 brochure.
Important Notification:
(2026-1-27) PEAQ-ITC Automatic system is available for service. Please refer to PEAQ-ITC sample submission guideline for sample preparation.
(2025-09-16) PEAQ-ITC Automatic system's thermal core can not response to the set power. Service is called. Sorry for the inconvenience.
(2025-07-22) PEAQ-ITC Automatic system is available for service. Please submit your requests through reservation system.
* Do not shake, bang or knock the bench durining experiments.
* Do not plug or withdraw USB in the middle of a run; this causes system error and the experiment will be aborted by the control program.
* Do not analyze the data file that is not finished. Instead, copy the data file and analyze the copied data.
* The process for cleaning the injector syringe is modified, please ask Dr. Jao for the correct procedure.
* Use only 200uL PCR tubes provided by BCF (Rainin Cat#3-TC02-N). Some PCR tubes (ABgene cat.# AB-0337) do not fit the holder and the injector syringe may hit the bottom of the tubes.
Selected Applications
(Bio)molecular interactions
Dissociations (Dimer)
Interactions with vesicles/micelles
Enzyme kinetics
Related Softwares
Specification
| Cell volume | 200 μl |
| Min. Sample requirement | 5 - 10 μg of protein |
| direct measure range (milli - nano) | 102 to 109 M-1 |
| competetive measure range (nano - pico) | 109 to 1012 M-1 |
Suggested Reading:
iTC200 Microcalorimeter User's Manual.
ITC Data Analysis in Origin Tutorial Guide.
Picchianti, Lorenzo, Arthur Sedivy, and Yasin Dagdas, 2023, Characterization of Atg8-Family Interactors by Isothermal Titration Calorimetry, In Plant Proteostasis: Methods and Protocols, Edited by L. Maria Lois and Marco Trujillo.
Freyer MW and Lewis EA, 2008, Isothermal titration calorimetry: experimental design, data analysis, and probing macromolecule/ligand binding and kinetic interactions, Methods in Cell Biology, 84, 79-113.
Bjelić S and Jelesarov I, 2008, A survey of the year 2007 literature on applications of isothermal titration calorimetry, J. Mol. Recognit., 21, 289-311.
Thomson JA, Ladbury JE, 2004, Isothermal titration calorimetry: A tutorial. In Biocalorimetry 2: Applications of calorimetry in the biological sciences, Ladbury JE, Doyle ML (eds). John Wiley & Sons Ltd.:Chichester, UK; pp.37-58.