Introduction
The Octet Red96 system is a biolayer interferometry biosensor for measuring protein concentration, the kinetics and affinity of intermolecular interactions. With the 96-welled microplate design, it is applicable for screening of protein-protein and protein-small molecules interaction.
For more detail, please visit Ocetet Red96 website.
Important Notification:
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(2022-04-15) Please consult BCF staff first if you intend to use different microplates (not Greiner 655209).
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(2022-03-28) The Octet RED96 is restored and available for reservation. M108 Training Course is scheduled to April 19th (Tue.), please register through BCF reservation system.
* (2021-11-17) If "critical error in motion control" occurs and the data is not saved properly, try checking "Ignore errors in files when loading" in the analysis software when processing.
* (2021-09-22) From time to time, an error message of “critical error of motion control” occurs. Although service is called, the cause of this error cannot be identified. However, it seems to happen at the first experiment. To get around it, please perform a dummy experiment with the method named “DummyEXP.fmf” before any serious experiments! The method file is in the Data folder.
* (2021-09-13) The heater plate of Octet RED96 is replaced, therefore the temperature control function is back to normal.
* (2021-08-03) The control PC of Octet RED96 is replaced.
* (2021-05-26) Octet RED96 sometimes showed message of "critical error of motion control" in the middle of an experiment especially when ejecting sensors. Service has been called. Please pay attention to the pop-up message when reserve this instrument! Sorry for the inconvenience.
* (2017-06-21) The Data Acqusition and Data Analysis softwares are both upgraded to version 10.0. Users can still access version 7.0 on the desktop.
Specification
| Sample volume | 180 - 220 μl |
| Orbital Flow Capacity | 100 - 1500 rpm |
| Automation | 8 biosensors in parallel |
| Sample/reagent capacity | 1x96-well plate |
| Temperature range | (Ambient + 4℃)–40 ℃ |
| Baseline noise | <=3 pm |
| ka | 1E1 - 1E7/Ms for proteins 1E3 - 1E6/Ms for small molecules |
| kd | 1E-6 - 0.1 s for proteins 1E-3 - 1 s for small molecules |
| Range for hIgG | 0.5~2000 µg/mL @200rpm
0.05~100 µg/mL @1000rpm |
| Minimum molecular weight for kinetics | >150Da |
For more detail, please refer to Octet System Data Acquisition Software User Guide.
Suggested Reading:
Octet System Data Acquisition Software User Guide
Octet Data Analysis Software 7.0 User Guide
Concepcion J. 2009 Label-free detection of biomolecular interactions using BioLayer interferometry for kinetic characterization. Comb Chem High Throughput Screen., 12, 791-800.
Ekiert et al., 2011 A Highly Conserved Neutralizing Epitope on Group 2 Influenza A Viruses, Science, 333, 843-850.
Martin et al., 2011, Novartis Evaluation of the ForteBio Octet RED: A Versatile Instrument for Direct Binding Experiments. In Label-Free Technologies for Drug Discovery, Matthew Cooper and Lorenz M. Mayr (eds). John Wiley & Sons Ltd.; pp.224-240.
Wartchow CA. 2011 Biosensor-based small molecule fragment screening with biolayer interferometry. J Comput Aided Mol Des. 25, 669-676.
Chris M. Maragos 2011 Detection of deoxynivalenol using biolayer interferometry Mycotoxin Res. 27, 157-165.