Introduction
VP-Capillary DSC is a differential scanning calorimeter (DSC), measuring the difference in the heat required to increase the temperature of a sample and its reference. This techinque is appliable for studies of phase transition, melting point and such transitions involve energy or heat capacity changes.
For more detail, please refer to VP-Cap DSC website.
Important Notification:
* (2016-11-01) Free VP-Cap DSC sample tests available for Sinica users only till 2016-12-31.
* (2018-08-01) Please check PEAQ-DSC page for sample submission.
Features
- Sample requirement ~ 400 μl of 0.2 - 1 mg/mL proteins.
- Non-reactive Tantalum 61™ capillary cells.
- Operating temperature range of 10ºC to 120ºC
(Ask BCF staff for temperatures outside this range). - Peltier element for precise temperature control.
- User selectable temperature scan rates, 0 to 200ºC per hour upscans.
- Pressurizing system (~60 psi).
Applications
- Protein stability and folding.
- Liquid biopharmaceutical formulations.
- Process development.
- Protein engineering.
- Antibody domain studies.
- Characterization of membranes, lipids, nucleic acids and micellar systems.
- Assessment of the effects of structural change on a molecule’s stability.
- Assessment of biocomparability during manufacturing.
Suggested Reading:
VP-Cap DSC Microcalorimeter User's Manual.
DSC Data Analysis in Origin Tutorial Guide.
Cooper A, Nutley MA, and Wadood A 2001, Differential scanning microcalorimetry In Protein-Ligand Interactions: hydrodynamics and calorimetry, Harding SE, Chowdhry BZ (eds). Oxford University Press; pp.287-318.