Introduction
The Monolith NT.115Pico system ultilizes MicroScale Thermophoresis technology (MST) to measure the changes of the mobility of fluorescently labelled molecules along a temperature gradient. The mobility of a molecule is dependent on its size, shape, charge and hydration shell. By adding serial dilution of non-labelled binding partner, the instrument obtains equilibrium binding constant, KD. Because of its high sensitivity, the quality of sample solutions is also easy to monitor.
For more detail, please visit NanoTemper website.
Important Notification:
* (2014-12-2) Do not open more than two files when using NTAnalysis. Quit the program before open the next file. Otherwise, reports generated for the second one will have a wrong CapScan file name.
Applications
Monolith NT.115pico is capable for the following studies:
Related Softwares
PALMIST for the analysis of microscale thermophoresis data.
Suggested Reading:
Monolith NT.115pico Quick Starting Guide
Monolith NT.115pico Manual
Dennis Breitsprecher & Alexey Rak et al., 2016, An automated microscale thermophoresis screening approach for fragment-based lead discovery, Journal of Biomolecular Screening, 21, 414–421.
Thomas H. Scheuermann & Chad A. Brautigam et al., 2016, On the acquisition and analysis of microscale thermophoresis data, Analytical Biochemistry, 496, 79-93.
Moran Jerabek-Willemsen & Dennis Breitsprecher et al., 2014, MicroScale Thermophoresis: Interaction analysis and beyond, Journal of Molecular Structure, 1077, 101-113.
Susanne A.I. Seidel & Stefan Duhr et al., 2013, Microscale thermophoresis quantifies biomolecular interactions under previously challenging conditions, Methods, 59, 301-315.
CJ Wienken & Stefan Duhr et al., 2010, Protein-binding assays in biological liquids using microscale thermophoresis, Nat Commun., 1, 100.