Introduction
Mass Photometry measures the interference between the light scattered by the moleule and the light reflected by the measurement surface. The signal is directly correlated with the true molecular mass. Given appropriate calibration standards, the molecular mass is measured in a wide range from 30kDa to 5MDa at single-molecule resolution.
Important Notification:
(2024-06-11) For unknow reasons, a smear was found at the left edge of the viewing window and gradually shifted towards the center. The instrument is out of service. Service is called. Sorry for the inconvenience.
(2024-04-17) The dust is cleared out of the way. Large image mode is ready to use.
(2023-12-12) There is a dark spot under large image size condition. It will not interfere the measurements. Service is called.
Specification
| Mass range | 30kDa - 5MDa |
| Concentrtion range | 100pM - 100nM |
| Resolution (FWHM) | 25kDa@66kDa; 60kDa@660kDa |
| Mass Precision | ± 2% |
| Mass error | ± 5% |
Suggested Reading:
Refeyn TwoMP Mass Photometer User Maual
Refeyn DiscoverMP User Maual
Refeyn AcquireMP User Maual
Di Wu and Grzegorz Piszczek, Standard Protocol for Mass Photometry Experiments, Eur Biophys J. 2021 May; 50(3-4): 403–409.
[Webinar] Measuring Molecules with Light-Prof. Philipp Kukura, University of Oxford