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title Check Courses.

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arrow User's Guideline
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arrow Simplified Protocol

BCF Access :

Reservation Procedure

Few probes for tests

* SA Probes
* Flex SA Probes
* Small Molecule Analysis Probes (SMAP)
* SA XT
* Streptactin XT
* Amine-Reactive Probes
* Ni-NTA Probes
* Anti-His Probes (HIS)
* Anti-GST Probes
* Anti-Human FAB Probes (FAB)
* Anti-Mouse IgG Fc Probes (MFC)
* Anti-Human IgG Fc Gen II probes (HFCII)
* Protein A Probes (ProA)

 

Introduction

The GatorPlus utilizes biolayer interferometry for measuring protein concentration, kinetics and affinity of intermolecular interactions. This instrument can accommodate standard 96-well or 384-well microplate, it is capable of screening of protein-protein and protein-small molecule interactions.

For more detail, please visit Gator plus website.

Important Notification:

(2022-11-16)  GatorPlus is ready to accept operator certification application. Until Jan. 31st, 2023, the certification is free and you are welcome to bring your own samples for the test.

title Specification

Plate capacity 96-well or 384-well
Throughput 1 to 8 samples in paralle,
1 to 456 samples per assay
Sample volume
180 μl (96-well plate)
40 μl (384-well plate)
Orbital speed
100-2000 rpm
Baseline noise
<=3 pm
Ka
1E1 - 1E6/MS
Kd
1E-6 - 0.1/s

For more detail, please refer to Gator Plus Label-Free Biosanalysis System User Manual.

title Suggested Reading:

Gator Plus Label-Free Biosanalysis System User Manual.

Gator Plus Label-Free Biosanalysis System User Manual.
Li et al., 2022, Broad neutralization of SARS-CoV-2 variants by an inhalable bispecific single-domain antibody, Cell, 185, 1389–1401. https://doi.org/10.1016/j.cell.2022.03.009